Enzymatic fragment condensation of side chain-protected peptides using subtilisin A in anhydrous organic solvents: a general strategy for industrial peptide synthesis

Nuijens, T., Schepers, A.H.M., Cusan, C., Kruijtzer, J.A.W., Rijkers, D.T.S., Liskamp, R.M.J. and Quaedflieg, P.J.L.M. (2013) Enzymatic fragment condensation of side chain-protected peptides using subtilisin A in anhydrous organic solvents: a general strategy for industrial peptide synthesis. Advanced Synthesis & Catalysis, 355(2-3), pp. 287-293. (doi: 10.1002/adsc.201200694)

Abstract

Herein, the enzymatic condensation of side chain-protected peptide fragments using subtilisin A in anhydrous organic solvents is described. A screening with dipeptide Cbz-Val-Xxx carboxamidomethyl esters with H-Xxx-Val-NH2 nucleophiles was performed, wherein Xxx stands for every (side chain-protected) amino acid residue. Finally, it was demonstrated that it is feasible to enzymatically condense larger peptide fragments (up to the 10-mer level) bearing multiple side chain-protecting groups with very high conversion.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Liskamp, Professor Robert
• Authors: Nuijens, T., Schepers, A.H.M., Cusan, C., Kruijtzer, J.A.W., Rijkers, D.T.S., Liskamp, R.M.J., and Quaedflieg, P.J.L.M.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Advanced Synthesis & Catalysis
• Publisher: Wiley
• ISSN: 1615-4150