GxcC connects rap and rac signaling during dictyostelium development

Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P.J.M. and Kortholt, A. (2013) GxcC connects rap and rac signaling during dictyostelium development. BMC Cell Biology, 14(6), (doi: 10.1186/1471-2121-14-6)

Abstract

Background: Rap proteins belong to the Ras family of small G-proteins. Dictyostelium RapA is essential and implicated in processes throughout the life cycle. In early development and chemotaxis competent cells RapA induces pseudopod formation by activating PI3K and it regulates substrate attachment and myosin disassembly via the serine/threonine kinase Phg2. RapA is also important in late development, however so far little is known about the downstream effectors of RapA that play a role in this process.

Results: Here we show that cells expressing constitutively active RapA exhibit a high level of Rac activation. With a pull-down screen coupled to mass spectrometry, we identified the Rac specific guanine nucleotide exchange factor, GxcC, as Rap binding partner. GxcC binds directly and specifically to active RapA and binds to a subset of Dictyostelium Rac proteins. Deletion studies revealed that this pathway is involved in regulating Dictyostelium development.

Conclusions: GxcC provides a novel link between Rap and Rac signalling and is one of the Rap effectors regulating the progression of multicellular development.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: UNSPECIFIED
• Authors: Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P.J.M., and Kortholt, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: BMC Cell Biology
• Publisher: Biomed Central
• ISSN: 1471-2121
• Copyright Holders: Copyright © 2013 The Authors
• First Published: First published in BMC Cell Biology 14(6)
• Publisher Policy: Reproduced under a Creative Commons License