A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Li, X. et al. (2009) A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex. FEBS Letters, 583(20), pp. 3310-3316. (doi: 10.1016/j.febslet.2009.09.035)

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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2009.09.035

Abstract

βarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/βarrestin signalling complex. We show that βarrestin 1 and βarrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of βarrestin 1 and βarrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the βarrestin 1 and βarrestin 2 we identify a novel MKK4 docking site.

Item Type:Articles
Keywords:Arrestin, JNK, scaffold, MKK4, ASK1, peptide array
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dunlop, Dr Allan and Gibson, Dr Lucien and Advant, Miss Noopur and Baillie, Professor George and Houslay, Professor Miles and Walsh, Nicola and MacLeod, Miss Ruth
Authors: Li, X., MacLeod, R., Dunlop, A. J., Edwards, H. V., Advant, N., Gibson, L. C.D., Devine, N. M., Brown, K. M., Adams, D. R., Houslay, M. D., and Baillie, G. S.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:FEBS Letters
Publisher:Elsevier BV
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:24 September 2009
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
438301Phosphodiesterase-4 isoforms - intracellular targeting, regulation and potential therapeutic targetsMiles HouslayMedical Research Council (MRC)G0600765Institute of Neuroscience and Psychology