Schiffrin, E.L., Larivière, R. and Touyz, R.M. (1995) ETA and ETB receptors on vascular smooth muscle cells from mesenteric vessels of SHR. Clinical and Experimental Pharmacology and Physiology, 22(s1), S193-S194. (doi: 10.1111/j.1440-1681.1995.tb02877.x)
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Abstract
1. To investigate the contribution of ET<sub>A</sub> and ET<sub>B</sub> receptors, calcium responses to the ET<sub>B</sub> agonist, IRL-1620, to endothelin-1 (ET-1) and to the ET<sub>A</sub> antagonist, BQ-123, were examined in primary cultured unpassaged vascular smooth muscle cells (VSMC) from mesenteric vessels of 3, 9 and 17 week old spontaneously hypertensive rats (SHR), Wistar and Wistar-Kyoto (WKY) rats using Fura-2 methodology. 2. IRL-1620 (10-7mol/L) and ET-1 (10-9mol/L) increased [Ca2+]i in all strains and ages. Responses to ET-1 and IRL-1620 were blunted in 17 week SHR. BQ-123 significantly reduced ET-1-stimulated [Ca2+]i. In endothelium-denuded mesenteric vessels, ET-1 and IRL-1620 induced significant [Ca2+]i responses. 3. Binding of ET-1 was significantly lower in mesenteric artery membranes from 17 week SHR compared to controls. 4. Thus, ET<sub>B</sub> receptors are present in rat mesenteric VSMC. In adult SHR, a reduced density of ET receptors results in decreased responses to IRL-1620 and to ET-1.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Touyz, Professor Rhian |
Authors: | Schiffrin, E.L., Larivière, R., and Touyz, R.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Journal Name: | Clinical and Experimental Pharmacology and Physiology |
ISSN: | 0305-1870 |
ISSN (Online): | 1440-1681 |
Published Online: | 28 June 2007 |
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