Touyz, R.M. , Tolloczko, B. and Schiffrin, E.L. (1995) Blunted attenuation of angiotensin II-mediated Ca2+ transients by insulin in cultured unpassaged vascular smooth muscle cells from spontaneously hypertensive rats. American Journal of Hypertension, 8(2), pp. 104-112. (doi: 10.1016/0895-7061(94)00173-9)
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Abstract
Insulin attenuates vasoconstrictor-stimulated intracellular calcium ([Ca2+]i) responses in cells from normotensive rats. To determine whether these effects may be altered in hypertension, this study assesses the effects of physiologic concentrations of insulin on angiotensin II-stimulated [Ca2+]i in primary unpassaged cultured vascular smooth muscle cells (VSMC) from mesenteric arteries from spontaneously hypertensive rats (SHR), Wistar Kyoto (WKY), and Wistar rats. [Ca2+]i was measured microphotometrically in cells from 3-, 9-, and 17-week-old rats by the Fura 2 methodology. Basal, angiotensin II-stimulated (1 nmol/L), and insulin-attenuated (70 microU/mL) angiotensin II-induced [Ca2+]i did not differ in VSMC obtained from 3-week-old WKY, Wistar, and SHR. Basal and angiotensin II-stimulated [Ca2+]i in VSMC from 9- and 17-week-old SHR was significantly greater (P < .01) than that in cells from age-matched WKY and Wistar rats. Insulin decreased angiotensin II-stimulated [Ca2+]i responses in all groups, but the effect was significantly blunted in cells from 9- and 17-week-old SHR. The magnitude of inhibition of angiotensin II-stimulated [Ca2+]i responses induced by insulin was 63 +/- 12 nmol/l (WKY), 60 +/- 10 nmol/L (Wistar), and 28 +/- 8 nmol/L (SHR), (P < .01 v normotensive) in cells from 9-week-old rats and 70 +/- 15 nmol/L (WKY), 67 +/- 12 nmol/L (Wistar), and 25 +/- 10 nmol/L (SHR) (P < .01 v normotensive) in cells from 17-week-old rats. Insulin increased angiotensin II-stimulated [Ca2+]i recovery rate to basal values in cells from WKY rats, but had no effect on the rate of recovery in VSMC from SHR.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Touyz, Professor Rhian |
| Authors: | Touyz, R.M., Tolloczko, B., and Schiffrin, E.L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | American Journal of Hypertension |
| ISSN: | 0895-7061 |
| ISSN (Online): | 1941-7225 |
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