Vitronectin activity on polymer substrates with controlled –OH density

Toromanov, G., Gonzalez-Garcia, C., Altankov, G. and Salmerón-Sánchez, M. (2010) Vitronectin activity on polymer substrates with controlled –OH density. Polymer, 51(11), pp. 2329-2336. (doi: 10.1016/j.polymer.2010.03.041)

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Vitronectin (VN) adsorption on a family of model substrates consisting of copolymers of ethyl acrylate and hydroxyl ethylacrylate in different ratios (to obtain a controlled surface density of –OH groups) was investigated by Atomic Force Microscopy (AFM). It is shown that the fraction of the substrate covered by the protein depends strongly on the amount of hydroxyl groups in the sample and it monotonically decreases as the –OH density increases. Isolated globular-like VN molecules are observed on the surfaces with the higher OH density. As the fraction of hydroxyl groups decreases, aggregates of 3–5 VN molecules are observed on the sample. Overall cell morphology, focal adhesion formation and actin cytoskeleton development are investigated to assess the biological activity of the adsorbed VN on the different surfaces. Dermal fibroblast cells show excellent material interaction on the more hydrophobic samples (OH contents lower than 0.5), which reveals enhanced VN activity on this family of substrates as compared with other extracellular matrix proteins (e.g., fibronectin and fibrinogen).

Item Type:Articles
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Gonzalez Garcia, Dr Cristina
Authors: Toromanov, G., Gonzalez-Garcia, C., Altankov, G., and Salmerón-Sánchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Polymer
ISSN (Online):1873-2291
Published Online:31 March 2010

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