Rapid and comprehensive evaluation of (poly)phenolic compounds in pomegranate (punica granatum L.) juice by UHPLC-MSn

Mena, P., Calani, L., Dall'Asta, C., Galaverna, G., García-Viguera, C., Bruni, R., Crozier, A. and Del Rio, D. (2012) Rapid and comprehensive evaluation of (poly)phenolic compounds in pomegranate (punica granatum L.) juice by UHPLC-MSn. Molecules, 17(12), pp. 14821-14840. (doi: 10.3390/molecules171214821)

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Abstract

The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Crozier, Professor Alan
Authors: Mena, P., Calani, L., Dall'Asta, C., Galaverna, G., García-Viguera, C., Bruni, R., Crozier, A., and Del Rio, D.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Molecules
ISSN:1420-3049
Published Online:13 December 2012
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Molecules 17(12):14821-14840
Publisher Policy:Reproduced under a Creative Commons License

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