Multilocus sequence typing of a global collection of pasteurella multocida isolates from cattle and other host species demonstrates niche association

Hotchkiss, E.J., Hodgson, J.C., Lainson, F.A. and Zadoks, R.N. (2011) Multilocus sequence typing of a global collection of pasteurella multocida isolates from cattle and other host species demonstrates niche association. BMC Microbiology, 11, 115. (doi: 10.1186/1471-2180-11-115) (PMID:21612618) (PMCID:PMC3120644)

Abstract

Background- Pasteurella multocida causes disease in many host species throughout the world. In bovids, it contributes to bovine respiratory disease (BRD) and causes haemorrhagic septicaemia (HS). Previous studies have suggested that BRD-associated P. multocida isolates are of limited diversity. A multilocus sequence typing (MLST) scheme for P. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method used, restricted sample selection or true niche association. Bovine respiratory isolates of P. multocida (n = 133) from the UK, the USA and France, collected between 1984 and 2008 from both healthy and clinically affected animals, were typed using MLST. Isolates of P. multocida from cases of HS, isolates from other host species and data from the MLST database were used as comparison. Results - Bovine respiratory isolates were found to be clonal (ISA 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins. Conclusions - The homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hotchkiss, Dr Emily and Zadoks, Professor Ruth
• Authors: Hotchkiss, E.J., Hodgson, J.C., Lainson, F.A., and Zadoks, R.N.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: BMC Microbiology
• Publisher: BioMed Central Ltd.
• ISSN: 1471-2180
• Published Online: 25 May 2011
• Copyright Holders: Copyright © 2011 The Authors
• First Published: First published in BMC Microbiology 11:115
• Publisher Policy: Reproduced under a Creative Commons License