Aguilar, A., Martinez, R., Camacho, F., Amin, N., Alfonso, J.L., Stott, D.I. and Perez, E.L. (2010) Ensayo de neutralización in vitro de aplicación en la evaluación de inmunógenos contra la hepatitis A, obtenidos mediante la tecnología de expresión en fagos filamentosos [In vitro neutralization assay to evaluate new hepatitis A vaccine candidates]. Vaccimonitor, 19(1), pp. 16-19.
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Publisher's URL: http://scielo.sld.cu/scielo.php?pid=S1025-028X2010000100003&script=sci_arttext
Abstract
The immunity against hepatitis A virus (HAV) is primarily due to the induction of neutralizing antibodies. Having neutralization assays is essential to evaluate new vaccine candidates against this pathogen. In this paper we developed a neutralization assay in vitro was used for evaluating the immunogenicity of HAV mimotopes obtained by phage display technology to be immunized BALB / c. Different dilutions of antibodies were incubated with 10 3 or 10 2 Dose Tissue Culture Infectious 50% (TCID 50 ) of clone cytopathic HM175/18f of HAV, and inoculated into plates with FRhK4 cells. After 7 days of incubation the neutralizing titer was determined as the reciprocal of the serum dilution capable of reducing the viral multiplication by 50%. Both 10 3 and 10 2 TCID 50 were neutralized by the monoclonal antibody 7E7 and human sera (A12 and A31) were positive for anti-HAV. Test Negative controls did not neutralize the virus. The neutralizing titers in the sera of mice immunized with mimotopes phage bearing HAV ranged between 4 and 16, whereas the preimmune sera from immunized mice and wild phage M13 did not neutralize viral infectivity. The neutralization assay was suitable for the evaluation of the immunogens proposed.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Stott I, Professor David |
| Authors: | Aguilar, A., Martinez, R., Camacho, F., Amin, N., Alfonso, J.L., Stott, D.I., and Perez, E.L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Vaccimonitor |
| ISSN: | 1025-028X |
| ISSN (Online): | 1025-0298 |
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