Stott, D.I. and McLearie, J. (1986) Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens. Immunological Investigations, 15(2), pp. 113-122. (doi: 10.3109/08820138609094137)
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Abstract
A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Stott I, Professor David |
| Authors: | Stott, D.I., and McLearie, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Immunological Investigations |
| ISSN: | 0882-0139 |
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