Patel, A.H. , Foster, T.J. and Pattee, P.A. (1989) Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4. Journal of General Microbiology, 135(7), pp. 1799-807. (doi: 10.1099/00221287-135-7-1799)
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Abstract
The gene coding for protein A (spa) has been mapped close to nov on the genetic map of the chromosome of Staphylococcus aureus 8325-4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the spa gene to be identified by blot +hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease SmaI fragment G was shown to carry the spa gene. An insertion mutation in spa was constructed by in vitro insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A spa::Kan(r)Neo(r) mutation was isolated in S. aureus 8325-4 by allele replacement. This provided a selectable marker which allowed the spa gene to be mapped by transformation analysis.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Patel, Professor Arvind |
| Authors: | Patel, A.H., Foster, T.J., and Pattee, P.A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of General Microbiology |
| Publisher: | Society for General Microbiology |
| ISSN: | 0022-1287 |
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