Tyler, J.K. and Everett, R.D. (1994) The DNA binding domains of the varicella-zoster virus gene 62 and herpes simplex virus type 1 ICP4 transactivator proteins heterodimerize and bind to DNA. Nucleic Acids Research, 22(5), pp. 711-721. (doi: 10.1093/nar/22.5.711)
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Abstract
The product of varicella-zoster virus gene 62 (VZV 140k) Is the functional counterpart of the major transcrlptional regulatory protein of herpes simplex virus type 1 (HSV-1), ICP4. We have found that the purified bacterlally expressed DNA blnding domain of VZV 140k (residues 417–647) is a stable dimer in solution. As demonstrated by the appearance of a novel protein -DNA complex of intermediate mobility in gel retardation assays, following In vitro co-translation of a pair of differently sized VZV 140k DNA binding domain peptides, the 140k DNA binding domain peptide binds to DNA as a dimer. In addition, the DNA binding domain peptides of HSV-1 ICP4 readily heterodimerizes with the VZV 140k peptide on co-translation, Indicating that HSV-1 ICP4 and VZV 140k possess very similar dimerization interfaces. It appears that only one fully wild type subunit of the dimer is sufficient to mediate sequence specific DNA recognition in certain circumstances. Co-lmmunopreclpitation analysis of mutant DNA binding domain peptides, co-translated with an epitope-tagged ICP4 DNA binding domain, shows that the sequence requirements for dimerlzation are lower than those necessary for DNA binding.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Everett, Professor Roger |
Authors: | Tyler, J.K., and Everett, R.D. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Nucleic Acids Research |
Publisher: | Oxford University Press |
ISSN: | 0305-1048 |
ISSN (Online): | 1362-4962 |
Copyright Holders: | Copyright © 1994 Oxford University Press |
First Published: | First published in Nucleic Acids Research 22(5):711-721 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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