Everett, R.D., Orr, A. and Elliott, M. (1991) High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110. Nucleic Acids Research, 19(22), pp. 6155-6161. (doi: 10.1093/nar/19.22.6155)
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Abstract
Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Everett, Professor Roger and Orr, Mrs Anne |
| Authors: | Everett, R.D., Orr, A., and Elliott, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Nucleic Acids Research |
| Publisher: | Oxford University Press |
| ISSN: | 0305-1048 |
| ISSN (Online): | 1362-4962 |
| Copyright Holders: | Copyright © 1991 Oxford University Press |
| First Published: | First published in Nucleic Acids Research 19(22):6155-6161 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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