Trans activation of plasmid-borne promoters by adenovirus and several herpes group viruses

Everett, R.D. and Dunlop, M. (1984) Trans activation of plasmid-borne promoters by adenovirus and several herpes group viruses. Nucleic Acids Research, 12(15), pp. 5969-5978. (doi: 10.1093/nar/12.15.5969)

Abstract

This paper describes experiments to test the ability of a number of viruses of the Herpes group, and also Adenovirus-2 and SV40, to activate transcription from the Herpes simplex virus-1 glycoprotein D and the rabbit β-globin promoters. Plasmids containing these genes were transfected into HeLa cells which were then infected with various viruses. Transcriptional activation in trans of the plasmid-borne promoters was monitored by quantitative S1 nuclease analysis of total cytoplasmic RNA isolated after infection. The results showed that Herpes simplex viruses 1 and 2, Pseudorabies virus, Variella Zoster virus, Human Cytomegalovirus, Equine herpes virus-1 and Adenovirus-2 activate transcription from both promoters tested. In contrast, SV40 did not activate transcription in trans in this assay. The possible mechanisms of this activation are discussed.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everett, Professor Roger
• Authors: Everett, R.D., and Dunlop, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Nucleic Acids Research
• Journal Abbr.: Nucl. Acids Res.
• ISSN: 0305-1048
• ISSN (Online): 1362-4962