Herpes simplex virus type 1 polypeptide ICP4 bends DNA

Everett, R.D., DiDonato, J., Elliott, M. and Muller, M. (1992) Herpes simplex virus type 1 polypeptide ICP4 bends DNA. Nucleic Acids Research, 20(6), pp. 1229-1233. (doi:10.1093/nar/20.6.1229)

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ICP4, the major regulatory polypeptide of herpes simplex virus type 1, is expressed at the earliest stages of virus infection and is required for the activation of transcription from the majority of viral promoters. It is a DNA binding protein which specifically recognises bipartite sites related to the sequence ATCGTnnnnnCGG. In this report we show that both partially purified ICP4, and its isolated DNA binding domain, bend DNA at occupied binding sites. The apparent angles of bend at two different binding sites were very similar and in both cases the centre of the bend was very close to the binding site sequence.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Everett, Professor Roger
Authors: Everett, R.D., DiDonato, J., Elliott, M., and Muller, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN (Online):1362-4962
Copyright Holders:Copyright © 1992 IRL Press at Oxford University Press
First Published:First published in Nucleic Acids Research 20(6):1229-1233
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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