DNA replication of bacteriophage T5. 3. Studies on the structure of concatemeric T5 DNA

Everett, R.D. (1981) DNA replication of bacteriophage T5. 3. Studies on the structure of concatemeric T5 DNA. Journal of General Virology, 52(1), pp. 25-38. (doi: 10.1099/0022-1317-52-1-25)

Abstract

The replication of bacteriophage T5 DNA has been shown to proceed via branched concatemeric intermediates. The structure of this concatemeric DNA was studied with respect to single-stranded regions and single-strand interruptions by digestion with S 1 nuclease and agarose gel electrophoresis after alkali denaturation. The results were compared with the pattern of'nicks' in the mature virion DNA, and the possible origins of these nicks are discussed. The structure of T5 concatemeric DNA was also studied by electron microscopy. Replication forks, loops and rare circular structures were observed, all of which were similar to those seen in replicating DNA of other large phages. Phage capsid structures were detected in association with both concatemeric and mature phage length DNA. These observations are discussed in relation to the replication, maturation and packaging of T5 DNA.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everett, Professor Roger
• Authors: Everett, R.D.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of General Virology
• Journal Abbr.: J. Gen. Virol.
• Publisher: Society for General Microbiology
• ISSN: 0022-1317
• ISSN (Online): 1465-2099