A 3′ co-terminus of two early herpes simplex virus type 1 mRNAs

McLauchlan, J. and Clements, J.B. (1982) A 3′ co-terminus of two early herpes simplex virus type 1 mRNAs. Nucleic Acids Research, 10(2), pp. 501-512. (doi: 10.1093/nar/10.2.501)

Abstract

A 3′ co-terminus of two early herpes simplex virus type 1 mRNAs has been identified using the nuclease-S1 mapping procedure with cloned virus DNA probes. These mRNAs (5.0 kb and 1.2 kb), located within the genome region 0.56-0.60, are unspliced and are transcribed rightwards on the prototype genome orientation. The position of their 3′ ends has been located on the virus DNA sequence and lies downstream from the polyadenylation signal 5′-AATAAA-3′. This hexanucleotide sequence also was present in the complementary DNA strand and was shown to be the polyadenylation signal for a leftwards-transcribed late mRNA. The abundance within the cytoplasm of the 5.0 kb and 1.2 kb mRNAs was investigated. Results indicated that these mRNAs were regulated in concert. It is suggested that sequences at the 3′ co-terminus may be involved in their regulation.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McLauchlan, Professor John
• Authors: McLauchlan, J., and Clements, J.B.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Nucleic Acids Research
• Journal Abbr.: Nucl. Acids Res.
• ISSN: 0305-1048
• ISSN (Online): 1362-4962