Davison, A.J. and Wilkie, N.M. (1983) Inversion of the two segments of the Herpes Simplex virus genome in intertypic recombinants. Journal of General Virology, 64(1), pp. 1-18. (doi: 10.1099/0022-1317-64-1-1)
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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-64-1-1
Abstract
We have analysed by restriction site mapping the structures of the termini and L-S joint in several HSV-1/HSV-2 intertypic recombinants, including Bx1(28-1), the virion DNA of which has a marked overabundance of one orientation of the L segment, and subclones of Bx1(28-1). All recombinants with both orientations of L present in equal amounts contain TRL and IRL regions derived at least in part from the same parent (HSV-1 or HSV-2) as a result of previously undetected crossovers in these regions. Recombinants with a predominance of one orientation of L have TRL and IRL regions derived from different parents. Homology between a sequences alone at the L terminus and L-S joint is sufficient for normal inversion of L. Analysis of another recombinant, RE4, which fails to invert normally in both L and S, suggests that normal inversion of S is dependent upon the presence of TRS and IRS regions derived at least in part from the same parent. We conclude that segment inversion specifically depends upon the a sequence, that the process of DNA replication and maturation does not necessarily produce molecules with identical a sequences, and that direct ligation of termini may occur during DNA replication.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Davison, Professor Andrew |
Authors: | Davison, A.J., and Wilkie, N.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Journal of General Virology |
Journal Abbr.: | J. Gen. Virol. |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1465-2099 |
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