Ord, E. N.J. , Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D. , Wilkinson, M., McCabe, C. , Macrae, I. M. and Work, L. M. (2012) Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats. Journal of Neuroscience Methods, 211(2), pp. 305-308. (doi: 10.1016/j.jneumeth.2012.09.001)
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Publisher's URL: http://dx.doi.org/10.1016/j.jneumeth.2012.09.001
Abstract
We describe a positive influence of pre-stroke surgery on recovery and survival in a commonly used experimental stroke model. Two groups of male, stroke-prone spontaneously hypertensive rats (SHRSPs) underwent transient middle cerebral artery occlusion (tMCAO). Group 1 underwent the procedure without any prior intervention whilst group 2 had an additional general anaesthetic 6 days prior to tMCAO for a cranial burrhole and durotomy. Post-stroke recovery was assessed using a 32 point neurological deficit score and tapered beam walk and infarct volume determined from haematoxylin–eosin stained sections. In group 2 survival was 92% (n = 12) versus 67% in group 1 (n = 18). In addition, post-tMCAO associated weight loss was significantly reduced in group 2. There was no significant difference between the two groups in experimental outcomes: infarct volume (Group 1 317 ± 18.6 mm<sup>3</sup> versus Group 2 332 ± 20.4 mm<sup>3</sup>), and serial (day 0–14 post-tMCAO) neurological deficit scores and tapered-beam walk test. Drilling a cranial burrhole under general anaesthesia prior to tMCAO in SHRSP reduced mortality and gave rise to infarct volumes and neurological deficits similar to those recorded in surviving Group 1 animals. This methodological refinement has significant implications for animal welfare and group sizes required for intervention studies.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Macrae, Professor Mhairi and Ord, Dr Emily and Wilkinson, Dr Michael and Van Kralingen, Ms Josie and Masson, Dr Rachel and McCabe, Dr Chris and Work, Dr Lorraine and McClure, Dr John |
Authors: | Ord, E. N.J., Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D., Wilkinson, M., McCabe, C., Macrae, I. M., and Work, L. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
Journal Name: | Journal of Neuroscience Methods |
Publisher: | Elsevier BV |
ISSN: | 0165-0270 |
ISSN (Online): | 1872-678X |
Published Online: | 10 September 2012 |
Copyright Holders: | Copyright © 2012 The Authors |
First Published: | First published in Journal of Neuroscience Methods 211(2):305-308 |
Publisher Policy: | Reproduced under a Creative Commons License |
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