Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

Orr, R.Y., Philip, N. and Waters, A.P. (2012) Improved negative selection protocol for Plasmodium berghei in the rodent malarial model. Malaria Journal, 11(103), (doi: 10.1186/1475-2875-11-103)

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An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Cameron, Mrs Rachael and Philip, Dr Nisha and Waters, Professor Andy
Authors: Orr, R.Y., Philip, N., and Waters, A.P.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Malaria Journal
Journal Abbr.:Malar. J.
Publisher:BioMed Central Ltd.
ISSN (Online):1475-2875
Published Online:31 March 2012
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Malaria Journal 11:103
Publisher Policy:Reproduced under a Creative Commons License

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