Tsimbouri, P.M. , McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P. , Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N. and Dalby, M.J. (2012) Using nanotopography and metabolomics to identify biochemical effectors of multipotency. ACS Nano, 6(11), pp. 10239-10249. (doi: 10.1021/nn304046m)
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Abstract
It is emerging that mesenchymal stem cell (MSC) metabolic activity may be a key regulator of multipotency. The metabolome represents a “snapshot” of the stem cell phenotype, and therefore metabolic profiling could, through a systems biology approach, offer and highlight critical biochemical pathways for investigation. To date, however, it has remained difficult to undertake unbiased experiments to study MSC multipotency in the absence of strategies to retain multipotency without recourse to soluble factors that can add artifact to experiments. Here we apply a nanotopographical systems approach linked to metabolomics to regulate plasticity and demonstrate rapid metabolite reorganization, allowing rational selection of key biochemical targets of self-renewal (ERK1/2, LDL, and Jnk). We then show that these signaling effectors regulate functional multipotency.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Tsimbouri, Dr Monica and Dalby, Professor Matthew and Alakpa, Miss Enateri and Reynolds, Dr Paul and Burgess, Dr Karl and Gadegaard, Professor Nikolaj |
| Authors: | Tsimbouri, P.M., McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P., Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N., and Dalby, M.J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Geographical and Earth Sciences |
| Journal Name: | ACS Nano |
| Publisher: | American Chemical Society |
| ISSN: | 1936-0851 |
| ISSN (Online): | 1936-086X |
| Published Online: | 16 October 2012 |
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