Ayllon, J., Russell, R.J., García-Sastre, A. and Hale, B.G. (2012) Contribution of NS1 effector domain dimerization to influenza A virus replication and virulence. Journal of Virology, 86(23), pp. 13095-13098. (doi: 10.1128/JVI.02237-12)
Full text not currently available from Enlighten.
Abstract
Conserved tryptophan-187 facilitates homodimerization of the influenza A virus NS1 effector domain. We generated a mutant influenza virus expressing NS1-W187R to destabilize this self-interaction. NS1-W187R protein exhibited lower dsRNA-binding activity, showed a temporal redistribution during infection, and was minimally compromised for interferon-antagonism. The mutant virus replicated similarly to wild-type in vitro, but was slightly attenuated for replication in mice, causing notably reduced morbidity/mortality. These data suggest biological relevance for the W187-mediated homotypic interaction of NS1.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hale, Dr Benjamin |
| Authors: | Ayllon, J., Russell, R.J., García-Sastre, A., and Hale, B.G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Virology |
| Journal Abbr.: | J. Virol. |
| ISSN: | 0022-538X |
| ISSN (Online): | 1098-5514 |
| Published Online: | 19 September 2012 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric