Intra- and interhost evolutionary dynamics of equine influenza virus

Murcia, P.R. et al. (2010) Intra- and interhost evolutionary dynamics of equine influenza virus. Journal of Virology, 84(14), pp. 6493-6454. (doi: 10.1128/JVI.00112-10)

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Determining the evolutionary basis of cross-species transmission and immune evasion is key to understanding the mechanisms that control the emergence of either new viruses or novel antigenic variants with pandemic potential. The hemagglutinin glycoprotein of influenza A viruses is a critical host range determinant and a major target of neutralizing antibodies. Equine influenza virus (EIV) is a significant pathogen of the horse that causes periodical outbreaks of disease even in populations with high vaccination coverage. EIV has also jumped the species barrier and emerged as a novel respiratory pathogen in dogs, canine influenza virus. We studied the dynamics of equine influenza virus evolution in horses at the intrahost level and how this evolutionary process is affected by interhost transmission in a natural setting. To this end, we performed clonal sequencing of the hemagglutinin 1 gene derived from individual animals at different times postinfection. Our results show that despite the population consensus sequence remaining invariant, genetically distinct subpopulations persist during the course of infection and are also transmitted, with some variants likely to change antigenicity. We also detected a natural case of mixed infection in an animal infected during an outbreak of equine influenza, raising the possibility of reassortment between different strains of virus. In sum, our data suggest that transmission bottlenecks may not be as narrow as originally perceived and that the genetic diversity required to adapt to new host species may be partially present in the donor host and potentially transmitted to the recipient host.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Murcia, Professor Pablo
Authors: Murcia, P.R., Baillie, G.J., Daly, J., Elton, D., Jervis, C., Mumford, J.A., Newton, R., Parrish, C.R., Hoelzer, K., Dougan, G., Parkhill, J., Lennard, N., Ormond, D., Moule, S., Whitwham, A., McCauley, J.W., McKinley, T.J., Holmes, E.C., Grenfell, B.T., and Wood, J.L.N.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
ISSN (Online):1098-5514
Published Online:05 May 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
579921Intra and inter-host evolution of equine and swine influenza virusesPablo MurciaWellcome Trust (WELLCOME)088494/Z/09/ZIII - CENTRE FOR VIRUS RESEARCH