Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers

Witte, C., Kremer, C., Cooper, J.M. and Neale, S.L. (2013) Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers. In: SPIE Photonics West MOEMS-MEMS, San Francisco, 2-7th Feb, (doi: 10.1117/12.2004814)

Abstract

A versatile platform for efficient cell lysis using a combination of acoustic and electric fields in a microchannel is presented. Cell membrane disruption is triggered by electric fields inducing electroporation and then lysis. The principle of optoelectronic tweezers (OET) is applied to control the electric field strength and a surface acoustic wave transducer is attached to an OET chip to implement acoustic tweezing (AT). The system is characterized in terms of spatial control of electric fields, single cell precision and lysis

• Item Type: Conference Proceedings
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Neale, Dr Steven and Witte, Dr Christian and Cooper, Professor Jonathan
• Authors: Witte, C., Kremer, C., Cooper, J.M., and Neale, S.L.
• Subjects: Q Science > Q Science (General)
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Copyright Holders: Copyright © 2013 Society of Photo Optical Instrumentation Engineers
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher