Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling

Kantawong, F., Burgess, K.E.V., Jayawardena, K., Hart, A., Burchmore, R.J. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling. Biomaterials, 30(27), pp. 4723-4731. (doi: 10.1016/j.biomaterials.2009.05.040)

Abstract

Topographic features can modulate cell behaviours such as proliferation, migration, differentiation and apoptosis. Biochemical mechanotransduction implies the conversion of mechanical forces (e.g. changes in cell spreading and morphology from changing surface topography) into biochemical signal via biomolecules. Still, little is known concerning which pathways may be directly involved in cell response to changes in the material surface. A number of pathways have been implicated using focused studies of [`]selected' biomolecules rather than a global analysis of signal pathways. This study used a controlled disorder nanopit topography (NSQ50, fabricated by electron beam lithography) to direct osteoblast differentiation of progenitor cells. This topography is unique as it represents a middle route (from absolute order or random roughness) that allows osteoconversion with similar efficiency as dexamethasone and ascorbate treatment. Two direct-comparison proteomics techniques, firstly gel-based and then chromatography-based, were used to analyse progenitor proteome changes in response to the nanotopography. Many of the changed proteins form part of the Extracellular Signal-regulated Kinase (ERK1/2) pathway

• Item Type: Articles
• Keywords: Tissue engineering, disordered topography, extracellular-activated protein kinase, osteoprogenitor cells, proteomics
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Burchmore, Dr Richard and Hart, Mr Andrew and Burgess, Dr Karl and Gadegaard, Professor Nikolaj and Dalby, Professor Matthew
• Authors: Kantawong, F., Burgess, K.E.V., Jayawardena, K., Hart, A., Burchmore, R.J., Gadegaard, N., Oreffo, R.O.C., and Dalby, M.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Engineering > Biomedical Engineering; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Biomaterials
• ISSN: 0142-9612
• Published Online: 26 June 2009