Sullivan, S. , Thompson, C. E., Kaiserli, E. and Christie, J. M. (2009) Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases. FEBS Letters, 583(13), pp. 2187-2193. (doi: 10.1016/j.febslet.2009.06.011) (PMID:19524572)
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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2009.06.011
Abstract
Phototropin receptor kinases play an important roles in optimising plant growth in response to blue light. Much is known regarding their photochemical reactivity, yet little progress has been made to identify downstream signalling components. Here, we isolated several interacting proteins for Arabidopsis phototropin 1 (phot1) by yeast two-hybrid screening. These include members of the NPH3/RPT2 (NRL) protein family, proteins associated with vesicle trafficking, and the 14-3-3 lambda (?) isoform from Arabidopsis . 14-3-3? and phot1 were found to colocalise and interact in vivo. Moreover, 14-3-3 binding to phot1 was limited to non-epsilon 14-3-3 isoforms and was dependent on key sites of receptor autophosphorylation. No 14-3-3 binding was detected for Arabidopsis phot2, suggesting that 14-3-3 proteins represent specific mode of phot1 signalling.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sullivan, Dr Stuart and Kaiserli, Dr Eirini and Christie, Professor John |
| Authors: | Sullivan, S., Thompson, C. E., Kaiserli, E., and Christie, J. M. |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | FEBS Letters |
| Publisher: | Elsevier |
| ISSN: | 0014-5793 |
| ISSN (Online): | 1873-3468 |
| Published Online: | 12 June 2009 |
| Copyright Holders: | Copyright © 2009 Federation of European Biochemical Societies |
| First Published: | First published in Febs Letters 583(13): 2187-2193 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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