Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M., Gould, G.W. and Bryant, N.J. (2009) Characterization of two distinct binding modes between syntaxin 4 and Munc18c. Biochemical Journal, 419(Pt3), pp. 655-660. (doi: 10.1042/BJ20082293) (PMID:19193195)
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Abstract
Interaction of SM (Sec1/Munc18) proteins with their cognate syntaxins represents an important regulatory mechanism of SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor)-mediated membrane fusion. Understanding the conserved mechanisms by which SM proteins function in this process has proved challenging, largely due to an apparent lack of conservation of binding mechanisms between different SM-syntaxin pairs. In the present study, we have identified a hitherto uncharacterized mode of binding between syntaxin 4 and Munc18c that is independent of the binding mode shown previously to utilize the N-terminal peptide of syntaxin 4. Our data demonstrate that syntaxin 4 and Munc18c interact via two distinct modes of binding, analogous to those employed by syntaxin 1a-Munc18a and syntaxin 16-Vps45p (vacuolar protein sorting 45). These data support the notion that all syntaxin/SM proteins bind using conserved mechanisms, and pave the way for the formulation of unifying hypotheses of SM protein function
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gould, Professor Gwyn and Bryant, Dr Nia and Kelly, Dr Sharon and Brandie, Ms Fiona and Kantidakis, Dr Theodoros |
| Authors: | Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M., Gould, G.W., and Bryant, N.J. |
| Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Biochemical Journal |
| Journal Abbr.: | Biochem. J. |
| Publisher: | Portland Press Ltd. |
| ISSN: | 0264-6021 |
| ISSN (Online): | 1470-8728 |
| Published Online: | 04 February 2009 |
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