Stegger, M., Andersen, P.S., Kearns, A., Pichon, B., Holmes, M.A., Edwards, G., Laurent, F., Teale, C., Skov, R. and Larsen, A.R. (2012) Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecALGA251. Clinical Microbiology and Infection, 18(4), pp. 395-400. (doi: 10.1111/j.1469-0691.2011.03715.x)
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Publisher's URL: http://dx.doi.org/10.1111/j.1469-0691.2011.03715.x
Abstract
The recent finding of a new mecA homologue, mecALGA251, with only 70% nucleotide homology to the conventional mecA gene has brought the routine testing for mecA as a confirmatory test for methicillin-resistant Staphylococcus aureus (MRSA) into question. A multiplex PCR was designed to differentiate mecALGA251 from the known mecA together with detection of lukF-PV and the spa gene fragments, enabling direct spa typing by sequencing of the PCR amplicons. The PCR analysis and subsequent spa typing were validated on a large collection (n = 185) of contemporary MRSA and methicillin-sensitive S. aureus isolates, including 127 isolates carrying mecALGA251. The mecALGA251 gene was situated in staphylococcal cassette chromosome mec type XI elements, and sequence variation within a 631-bp fragment of mecALGA251 in 79 isolates indicated a very conserved gene sequence. Following a successful validation, the multiplex PCR strategy was implemented in the routine testing of MRSA for national surveillance. Over a 2-month period, among 203 samples tested, 12 new MRSA cases caused by isolates carrying mecALGA251 were identified, emphasizing the clinical importance of testing for these new MRSA isolates.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Edwards, Dr Giles |
| Authors: | Stegger, M., Andersen, P.S., Kearns, A., Pichon, B., Holmes, M.A., Edwards, G., Laurent, F., Teale, C., Skov, R., and Larsen, A.R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Clinical Microbiology and Infection |
| ISSN: | 1198-743X |
| ISSN (Online): | 1469-0691 |
| Published Online: | 19 March 2012 |
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