James, A.B. , Syed, N.H., Bordage, S., Marshall, J., Nimmo, G.M., Jenkins, G.I. , Herzyk, P. , Brown, J.W.S. and Nimmo, H.G. (2012) Alternative splicing mediates responses of the arabidopsis circadian clock to temperature changes. Plant Cell, 24(3), pp. 961-981. (doi: 10.1105/tpc.111.093948) (PMID:22408072) (PMCID:PMC3336117)
|
Text
62659.pdf - Published Version 1MB |
Abstract
Alternative splicing plays crucial roles by influencing the diversity of the transcriptome and proteome and regulating protein structure/function and gene expression. It is widespread in plants, and alteration of the levels of splicing factors leads to a wide variety of growth and developmental phenotypes. The circadian clock is a complex piece of cellular machinery that can regulate physiology and behavior to anticipate predictable environmental changes on a revolving planet. We have performed a system-wide analysis of alternative splicing in clock components in Arabidopsis thaliana plants acclimated to different steady state temperatures or undergoing temperature transitions. This revealed extensive alternative splicing in clock genes and dynamic changes in alternatively spliced transcripts. Several of these changes, notably those affecting the circadian clock genes LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO RESPONSE REGULATOR7, are temperature-dependent and contribute markedly to functionally important changes in clock gene expression in temperature transitions by producing nonfunctional transcripts and/or inducing nonsense-mediated decay. Temperature effects on alternative splicing contribute to a decline in LHY transcript abundance on cooling, but LHY promoter strength is not affected. We propose that temperature-associated alternative splicing is an additional mechanism involved in the operation and regulation of the plant circadian clock.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Nimmo, Dr Gillian and Nimmo, Professor Hugh and James, Dr Allan and Herzyk, Dr Pawel and Jenkins, Professor Gareth |
| Authors: | James, A.B., Syed, N.H., Bordage, S., Marshall, J., Nimmo, G.M., Jenkins, G.I., Herzyk, P., Brown, J.W.S., and Nimmo, H.G. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Plant Cell |
| Publisher: | American Society of Plant Biologists |
| ISSN: | 1040-4651 |
| ISSN (Online): | 1532-298X |
| Published Online: | 09 March 2012 |
| Copyright Holders: | Copyright © 2012 American Society of Plant Biologists |
| First Published: | First published in Plant Cell 24(3):961-981 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information