De Ciuceis, C., Amiri, F., Brassard, P., Endemann, D.H., Touyz, R.M. and Schriffin, E.L. (2005) Reduced vascular remodeling, endothelial dysfunction, and oxidative stress in resistance arteries of Angiotensin II-infused macrophage colony-stimulating factor-deficient mice: evidence for a role in inflammation in Angiotensin-induced vascular injury. Arteriosclerosis, Thrombosis, and Vascular Biology, 25(10), pp. 2106-2113. (doi: 10.1161/01.ATV.0000181743.28028.57)
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Publisher's URL: http://dx.doi.org/10.1161/01.ATV.0000181743.28028.57
Abstract
<b>Objective—</b> Angiotensin (Ang) II-induced vascular damage may be partially mediated by reactive oxygen species generation and inflammation. Homozygous osteopetrotic mice (Op/Op), deficient in macrophage colony-stimulating factor (m-CSF), exhibit reduced inflammation. We therefore investigated Ang II effects on vascular structure, function, and oxidant stress generation in this model.<p></p> <b>Methods and Results—</b> Adult Op/Op, heterozygous (Op/+), and wild type (+/+) mice underwent 14-day Ang II (1000 ng/kg per minute) or saline infusion. Blood pressure (BP) was assessed by radiotelemetry, mesenteric resistance artery vascular reactivity was studied on a pressurized myograph, and vascular superoxide and NAD(P)H oxidase activity by lucigenin chemiluminescence. Ang II increased BP in Op/+ and +/+ mice but not in Op/Op. Ang II-treated Op/+ and +/+ mice showed reduced acetylcholine-mediated relaxation (maximal relaxation, respectively, 64% and 67% versus 84% and 93% in respective controls; P<0.05), which was unaffected by l-NAME. Ang II-infused Op/Op mice arteries showed significantly less endothelial dysfunction than vehicle-infused counterparts (maximal relaxation 87% versus 96% in shams). Resistance arteries from Ang II-infused +/+ and Op/+ mice had significantly increased media-to-lumen ratio and media thickness, neither of which was altered in Op/Op mice compared with untreated littermates. Vascular media cross-sectional area, NAD(P)H oxidase activity and expression, and vascular cell adhesion molecule (VCAM)-1 expression were significantly increased by Ang II only in +/+ mice (P<0.05).<p></p> <b>Conclusions—</b> m-CSF–deficient mice (Op/Op) developed less endothelial dysfunction, vascular remodeling, and oxidative stress induced by Ang II than +/+ littermates, suggesting a critical role of m-CSF and proinflammatory mediators in Ang II-induced vascular injury.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Touyz, Professor Rhian |
Authors: | De Ciuceis, C., Amiri, F., Brassard, P., Endemann, D.H., Touyz, R.M., and Schriffin, E.L. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Journal Name: | Arteriosclerosis, Thrombosis, and Vascular Biology |
Publisher: | American Heart Association |
ISSN: | 1079-5642 |
ISSN (Online): | 1524-4636 |
Published Online: | 11 August 2005 |
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