Hutchison, S., Benson, R.A. , Gibson, V.B., Pollock, A.H., Garside, P. and Brewer, J.M. (2012) Antigen depot is not required for alum adjuvanticity. FASEB Journal, 26(3), pp. 1272-1279. (doi: 10.1096/fj.11-184556) (PMID:22106367) (PMCID:PMC3289510)
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Publisher's URL: http://dx.doi.org/10.1096/fj.11-184556
Abstract
Alum adjuvants have been in continuous clinical use for more than 80 yr. While the prevailing theory has been that depot formation and the associated slow release of antigen and/or inflammation are responsible for alum enhancement of antigen presentation and subsequent T- and B-cell responses, this has never been formally proven. To examine antigen persistence, we used the chimeric fluorescent protein EαGFP, which allows assessment of antigen presentation in situ, using the Y-Ae antibody. We demonstrate that alum and/or CpG adjuvants induced similar uptake of antigen, and in all cases, GFP signal did not persist beyond 24 h in draining lymph node antigen-presenting cells. Antigen presentation was first detectable on B cells within 6–12 h of antigen administration, followed by conventional dendritic cells (DCs) at 12–24 h, then finally plasmacytoid DCs at 48 h or later. Again, alum and/or CpG adjuvants did not have an effect on the magnitude or sequence of this response; furthermore, they induced similar antigen-specific T-cell activation in vivo. Notably, removal of the injection site and associated alum depot, as early as 2 h after administration, had no appreciable effect on antigen-specific T- and B-cell responses. This study clearly rules out a role for depot formation in alum adjuvant activity.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Brewer, Professor James and Garside, Professor Paul and Benson, Dr Robert and Hutchison, Dr Sharon and Gibson, Miss Vivienne |
Authors: | Hutchison, S., Benson, R.A., Gibson, V.B., Pollock, A.H., Garside, P., and Brewer, J.M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | FASEB Journal |
ISSN: | 0892-6638 |
ISSN (Online): | 1530-6860 |
Published Online: | 21 November 2011 |
Copyright Holders: | Copyright © 2012 The Authors |
First Published: | First published in FASEB Journal 2012 26(3):1272-1279 |
Publisher Policy: | Distributed under the terms of the Creative Commons Attribution Non-Commercial License |
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