Timmins, J., Schoehn, G., Kohlhaas, C.F., Klenk, H.D., Ruigrok, R.W. and Weissenhorn, W. (2003) Oligomerization and polymerization of the filovirus matrix protein VP40. Virology, 312(2), pp. 359-368. (doi: 10.1016/S0042-6822(03)00260-5)
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Publisher's URL: http://dx.doi.org/10.1016/S0042-6822(03)00260-5
Abstract
The matrix protein VP40 from Ebola virus plays an important role in the assembly process of virus particles by interacting with cellular factors, cellular membranes, and the ribonuclearprotein particle complex. Here we show that the N-terminal domain of VP40 folds into a mixture of two different oligomeric states in vitro, namely hexameric and octameric ringlike structures, as detected by gel filtration chromatography, chemical cross-linking, and electron microscopy. Octamer formation depends largely on the interaction with nucleic acids, which in turn confers in vitro SDS resistance. Refolding experiments with a nucleic acid free N-terminal domain preparation reveal a mostly dimeric form of VP40, which is transformed into an SDS resistant octamer upon incubation with E. coli nucleic acids. In addition, we demonstrate that the N-terminal domain of Marburg virus VP40 also folds into ringlike structures, similar to Ebola virus VP40. Interestingly, Marburg virus VP40 rings reveal a high tendency to polymerize into rods composed of stacked rings. These results may suggest distinct roles for different oligomeric forms of VP40 in the filovirus life cycle
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Akehurst, Dr Christine |
Authors: | Timmins, J., Schoehn, G., Kohlhaas, C.F., Klenk, H.D., Ruigrok, R.W., and Weissenhorn, W. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Virology |
Publisher: | Elsevier |
ISSN: | 0042-6822 |
ISSN (Online): | 1096-0341 |
Published Online: | 20 June 2003 |
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