Vieyres, G., Angus, A.G.N., Haberstroh, A., Baumert, T.F., Dubuisson, J. and Patel, A.H. (2009) Rapid synchronization of hepatitis C virus infection by magnetic adsorption. Journal of Virological Methods, 157(1), pp. 69-79. (doi: 10.1016/j.jviromet.2008.11.015) (PMID:19100780)
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Abstract
Hepatitis C virus (HCV) entry into target cells is thought to be a multistep process involving several cellular factors. However, their precise role during virus entry is unclear. investigation of the mechanisms of HCV entry, such as the order of intervention by the cellular receptors, requires synchronizing infections. This study describes a new method involving magnetic adsorption of virus to nanoparticles to synchronize infection, which can be adapted to both HCV pseudoparticles and cell culture infectious HCV. By combining these particles with negatively or positively charged magnetic nanoparticles it was possible to adsorb them onto target cells under a magnetic field in only 2 min. This resulted in greater efficiency of virus adsorption to cells, and increased the infectivity of cell culture infectious virus, as compared to the standard protocol involving incubation of the virus with cells at 4°C for 1 h, or to a standard infection at 37°C. Furthermore, magnetic adsorption respected the natural entry route of the virus, making this system suitable to study the early stages of HCV infection.
| Item Type: | Articles |
|---|---|
| Keywords: | HCV; synchronization; adsorption; magnetic particles; neutralization |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Angus, Dr Allan and Patel, Professor Arvind |
| Authors: | Vieyres, G., Angus, A.G.N., Haberstroh, A., Baumert, T.F., Dubuisson, J., and Patel, A.H. |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Virological Methods |
| Publisher: | Elsevier |
| ISSN: | 0166-0934 |
| ISSN (Online): | 1879-0984 |
| Published Online: | 07 January 2009 |
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