Hale, B.G. , Knebel, A., Botting, C.H., Galloway, C.S., Precious, B.L., Jackson, D., Elliott, R.M. and Randall, R.E. (2009) CDK/ERK-mediated phosphorylation of the human influenza A virus NS1 protein at threonine-215. Virology, 383(1), pp. 6-11. (doi: 10.1016/j.virol.2008.10.002)
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Abstract
Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early viral propagation, an effect apparently unrelated to NS1 antagonizing host interferon responses or activating phosphoinositide 3-kinase signaling. In vitro, a subset of cellular proline-directed kinases, including cyclin dependent kinases (CDKs) and extracellular signal-regulated kinases (ERKs), potently phosphorylated NS1 protein at threonine-215. Our data suggest that CDK/ERK-mediated phosphorylation of NS1 at threonine-215 is important for efficient virus replication.
| Item Type: | Articles |
|---|---|
| Keywords: | Influenza |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Elliott, Professor Richard and Hale, Dr Benjamin |
| Authors: | Hale, B.G., Knebel, A., Botting, C.H., Galloway, C.S., Precious, B.L., Jackson, D., Elliott, R.M., and Randall, R.E. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH345 Biochemistry Q Science > QR Microbiology > QR355 Virology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Virology |
| ISSN: | 0042-6822 |
| ISSN (Online): | 1096-0341 |
| Published Online: | 13 November 2008 |
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