Chalmers, S., Caldwell, S. T. , Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G. and Hartley, R. C. (2012) Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society, 134(2), pp. 758-761. (doi: 10.1021/ja2077922) (PMID:22239373) (PMCID:PMC3260739)
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Abstract
Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Caldwell, Dr Stuart and Hartley, Professor Richard |
Authors: | Chalmers, S., Caldwell, S. T., Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G., and Hartley, R. C. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Journal of the American Chemical Society |
Publisher: | American Chemical Society |
ISSN: | 0002-7863 |
ISSN (Online): | 1520-5126 |
Published Online: | 22 December 2011 |
Copyright Holders: | Copyright © 2011 American Chemical Society |
First Published: | First published in Journal of the American Chemical Society 134(2):758-761 |
Publisher Policy: | Reproduced under a Creative Commons License |
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