Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Chalmers, S., Caldwell, S. T. , Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G. and Hartley, R. C. (2012) Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society, 134(2), pp. 758-761. (doi:10.1021/ja2077922) (PMID:22239373) (PMCID:PMC3260739)

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Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Caldwell, Dr Stuart and Hartley, Professor Richard
Authors: Chalmers, S., Caldwell, S. T., Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G., and Hartley, R. C.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Journal of the American Chemical Society
Publisher:American Chemical Society
ISSN:0002-7863
ISSN (Online):1520-5126
Published Online:22 December 2011
Copyright Holders:Copyright © 2011 American Chemical Society
First Published:First published in Journal of the American Chemical Society 134(2):758-761
Publisher Policy:Reproduced under a Creative Commons License

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