An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Hasenkamp, S., Wong, E.H. and Horrocks, P. (2012) An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum. Malaria Journal, 11(42), (doi: 10.1186/1475-2875-11-42)

Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Wong, Dr Eleanor
• Authors: Hasenkamp, S., Wong, E.H., and Horrocks, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Malaria Journal
• Publisher: Biomed Central
• ISSN: 1475-2875
• Published Online: 10 February 2012
• Copyright Holders: Copyright © 2012 The Authors
• First Published: First published in Malaria Journal 11:42
• Publisher Policy: Reproduced under a Creative Commons Licence