Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample

Dienerowitz, M., Gibson, G. , Bowman, R. and Padgett, M. (2011) Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample. Optics Express, 19(24), pp. 24589-24595. (doi: 10.1364/OE.19.024589)

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Publisher's URL: http://dx.doi.org/10.1364/OE.19.024589


We investigate the effects of 1st order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1μm polystyrene beads up to 50μm deep in the sample.<p></p>

Item Type:Articles
Glasgow Author(s) Enlighten ID:Gibson, Dr Graham and Padgett, Professor Miles and Dienerowitz, Dr Maria
Authors: Dienerowitz, M., Gibson, G., Bowman, R., and Padgett, M.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN (Online):1094-4087
Published Online:01 January 2011
Copyright Holders:Copyright © 2011 Optical Society of America
First Published:First published in Optics Express 19(24):24589-24595
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
482571Multi-object, high-throughput, spectro-microscopy: Life Sciences InterfaceMiles PadgettEngineering & Physical Sciences Research Council (EPSRC)EP/H007636/1Physics and Astronomy