Jamieson, S. , Barraclough, R. and Rudland, P.S. (1990) Transfection of a nonmetastatic diploid rat mammary epithelial cell line with the oncogenes for EJ-ras-1 and Polyoma large T-antigen. International Journal of Cancer, 46(6), pp. 1071-1080. (doi: 10.1002/ijc.2910460621)
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Abstract
Transfection of rat mammary (Rama) 37 epithelial cells which yield non-metastasizing adenomas in syngeneic Wistar-Furth rats with a drug resistance plasmid containing both the <i>neo</i> gene and EJ-<i>ras</i>-I (pSV2<i>neo-ras</i>) or with pSV2<i>neo</i> and a plasmid encoding the large T Antigen (pLT214) of polyoma virus yields drug-resistant transformants with a frequency of <sup>10−5</sup>. Representative transformants have been propagated in neo-selecting medium to yield various cell lines. The 7 lines transfected with pSV2<i>neo-ras</i> (EJI set) and the 10 lines cotransfected with pSV2<i>neo</i> and pLT214 (LTI set) all produce tumours at subcutaneous (s. c.) sites with a shorter median latent period than tumours produced by the parental Rama 37 cells. In addition, the LTI set: of transformants yields a higher incidence of tumours than the Rama 37 cells. No metastases are produced when any of the oncogene transformants are inoculated s. c. into rats. However, when an EJi representative is inoculated intravenously (i. v.), tumour deposits are found in the lungs of the host animals. In contrast, other Rama 37 variants that metastasize from s. c. sites fail to produce any metastases when inoculated i. v. The oncogene transfectants contain integrated DNA that hybridizes to neo and to the requisite oncogenic DNAs; the pattern of hybridizing bands to the transfected genes and their expression as mRNA is complex, and is presented in detail.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Jamieson, Professor Susan |
| Authors: | Jamieson, S., Barraclough, R., and Rudland, P.S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities |
| Journal Name: | International Journal of Cancer |
| Journal Abbr.: | Int. J. Cancer |
| ISSN: | 0020-7136 |
| ISSN (Online): | 1097-0215 |
| Published Online: | 17 July 2006 |
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