Kantawong, F., Burchmore, R.J.S., Wilkinson, C.D.W., Oreffo, R.O.C. and Dalby, M.J. (2009) Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance. Acta Biomaterialia, 5(4), pp. 1137-1146. (doi: 10.1016/j.actbio.2008.11.001)
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Publisher's URL: http://dx.doi.org/10.1016/j.actbio.2008.11.001
Abstract
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype
Item Type: | Articles |
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Keywords: | Tissue engineering, Surface topography, Contact guidance, Osteoprogenitor cells, Proteomics |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and Wilkinson, Professor Christopher and Dalby, Professor Matthew |
Authors: | Kantawong, F., Burchmore, R.J.S., Wilkinson, C.D.W., Oreffo, R.O.C., and Dalby, M.J. |
Subjects: | R Medicine > R Medicine (General) Q Science > QP Physiology |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Acta Biomaterialia |
ISSN: | 1742-7061 |
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