Regulations of RNA Polymerase III transcription by Maf1 in mammalian cells

Goodfellow, S.J., Graham, E.L., Kantidakis, T., Marshall, L., Coppins, B.A., Oficjalska-Pham, D., Gérard, M., Lefebvre, O. and White, R.J. (2008) Regulations of RNA Polymerase III transcription by Maf1 in mammalian cells. Journal of Molecular Biology, 378(3), pp. 481-491. (doi: 10.1016/j.jmb.2008.02.060)

Abstract

RNA polymerase (pol) III produces essential components of the biosynthetic machinery; therefore, its output is tightly coupled with the rate of cell growth and proliferation. In <i>Saccharomyces cerevisiae</i>, Maf1 is an essential mediator of pol III repression in response to starvation. We demonstrate that a Maf1 ortholog is also used to restrain pol III activity in mouse and human cells. Mammalian Maf1 represses pol III transcription <i>in vitro</i> and in transfected fibroblasts. Furthermore, genetic deletion of Maf1 elevates pol III transcript expression, thus confirming the role of endogenous Maf1 as an inhibitor of mammalian pol III output. Maf1 is detected at chromosomal pol III templates in rodent and human cells. It interacts with pol III as well as its associated initiation factor TFIIIB and is phosphorylated in a serum-sensitive manner <i>in vivo</i>. These aspects of Maf1 function have been conserved between yeast and mammals and are therefore likely to be of fundamental importance in controlling pol III transcriptional activity

• Item Type: Articles
• Keywords: Maf1, RNA polymerase III, TFIIIB, transcription
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: White, Prof Robert and Kantidakis, Dr Theodoros
• Authors: Goodfellow, S.J., Graham, E.L., Kantidakis, T., Marshall, L., Coppins, B.A., Oficjalska-Pham, D., Gérard, M., Lefebvre, O., and White, R.J.
• Subjects: Q Science > QH Natural history > QH345 Biochemistry; Q Science > QH Natural history > QH426 Genetics
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Journal of Molecular Biology
• ISSN: 0022-2836