Higon, M. et al. (2011) Screening trematodes for novel intervention targets: a proteomic and immunological comparison of Schistosoma haematobium, Schistosoma bovis and Echinostoma caproni. Parasitology, 138(12), pp. 1607-1619. (doi: 10.1017/S0031182011000412)
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Publisher's URL: http://dx.doi.org/10.1017/S0031182011000412
Abstract
With the current paucity of vaccine targets for parasitic diseases, particularly those in childhood, the aim of this study was to compare protein expression and immune cross-reactivity between the trematodes <i>Schistosoma haematobium</i>, <i>S. bovis</i> and <i>Echinostoma caproni</i> in the hope of identifying novel intervention targets. Native adult parasite proteins were separated by 2-dimensional gel electrophoresis and identified through electrospray ionisation tandem mass spectrometry to produce a reference gel. Proteins from differential gel electrophoresis analyses of the three parasite proteomes were compared and screened against sera from hamsters infected with <i>S. haematobium</i> and <i>E. caproni</i> following 2-dimensional Western blotting. Differential protein expression between the three species was observed with circa 5% of proteins from <i>S. haematobium</i> showing expression up-regulation compared to the other two species. There was 91% similarity between the proteomes of the two S <i>chistosoma</i> species and 81% and 78.6% similarity between <i>S. haematobium</i> and <i>S. bovis</i> versus <i>E. caproni</i>, respectively. Although there were some common cross-species antigens, species-species targets were revealed which, despite evolutionary homology, could be due to phenotypic plasticity arising from different host-parasite relationships. Nevertheless, this approach helps to identify novel intervention targets which could be used as broad-spectrum candidates for future use in human and veterinary vaccines.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard |
| Authors: | Higon, M., Cowan, G., Nausch, N., Cavanagh, D., Oleaga, A., Toledo, R., Stothard, J.R., Antunez, O., Marcilla, A., Burchmore, R.J.S., and Mutapi, F. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Parasitology |
| Publisher: | Cambridge University Press |
| ISSN: | 0031-1820 |
| ISSN (Online): | 1469-8161 |
| Published Online: | 10 June 2011 |
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