Molecular analysis of pheochromocytoma after maternal transmission of SDHD mutation elucidates mechanism of parent-of-origin effect

Yeap, P. M. et al. (2011) Molecular analysis of pheochromocytoma after maternal transmission of SDHD mutation elucidates mechanism of parent-of-origin effect. Journal of Clinical Endocrinology and Metabolism, 96(12), E2009-E2013. (doi: 10.1210/jc.2011-1244)

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<b>Context:</b> Pheochromocytoma/paraganglioma occurs almost exclusively after paternal transmission of succinate dehydrogenase D (SDHD) mutations. This parent-of-origin effect has not been fully explained but is accompanied by obligate loss of the maternal copy of chromosome 11. Loss of wild-type SDHD and an additional imprinted gene (hypothesized to be H19) appears necessary for tumor formation. Two previous reports suggested tumor formation after maternal transmission of SDHD mutation, but histological and molecular characterization was unavailable.<p></p> <b>Objective:</b> We report the first kindred in which histologically confirmed pheochromocytoma/ paraganglioma occurred after maternal transmission of an SDHD mutation and investigate the molecular mechanism of tumor formation.<p></p> <b>Design:</b> The design of the investigation was the study of a three-generation family with SDHD c.242C>T (p.Pro81Leu) mutation.<p></p> <b>Results:</b> The index patient had a histologically confirmed pheochromocytoma and an identical SDHDgermline mutation (p.Pro81Leu) to her mother (who had a glomus jugulare tumor) and paraganglioma tissue from her maternal grandfather. Tumor DNA from the index patient revealed loss of heterozygosity (LOH) at 11q23, causing loss of the wild-type paternal SDHD allele and LOH affecting maternal 11p15, including H19. These two regions of LOH were separated by a region exhibiting clearly retained heterozygosity, including SDHAF2, a recently reported paraganglioma susceptibility gene.<p></p> <b>Conclusions:</b> Tumor formation can occur after maternal transmission of SDHD, a finding with important clinical implications for SDHD families. Tumor formation in SDHD mutation requires the loss of both the wild-type SDHD allele and maternal 11p15, leading to the predominant but now not exclusive pattern of disease inheritance after paternal SDHD transmission.<p></p>

Item Type:Articles
Additional Information:Yeap and Tobias were joint first authors.
Glasgow Author(s) Enlighten ID:Yeap, Miss Phey and Freel, Dr Marie and Lindsay, Dr Robert and Perry, Dr Colin and Fletcher, Mr Alexander and Cooke, Dr Alexander and Tobias, Professor Edward
Authors: Yeap, P. M., Tobias, E. S., Mavraki, E., Fletcher, A., Bradshaw, N., Freel, E. M., Cooke, A., Murday, V. A., Davidson, H. R., Perry, C. G., and Lindsay, R. S.
Subjects:R Medicine > RC Internal medicine
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
Journal Name:Journal of Clinical Endocrinology and Metabolism
Journal Abbr.:JCEM
Publisher:Endocrine Society
ISSN (Online):1945-7197
Published Online:21 September 2011
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
496251Translational studies on the role of corticosteroids in cardiovascular diseaseMarie FreelMedical Research Council (MRC)G0802803Institute of Cardiovascular and Medical Sciences