Morphological events during the cell cycle of Leishmania major

Ambit, A., Woods, K. L., Cull, B., Coombs, G. H. and Mottram, J. C. (2011) Morphological events during the cell cycle of Leishmania major. Eukaryotic Cell, 10(11), pp. 1429-1438. (doi: 10.1128/EC.05118-11)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1128/EC.05118-11

Abstract

The morphological events involved in the Leishmania major promastigote cell cycle have been investigated in order to provide a detailed description of the chronological processes by which the parasite replicates its set of single-copy organelles and generates a daughter cell. Immunofluorescent labelling of {beta}-tubulin was used to follow the dynamics of the subcellular cytoskeleton and to monitor the division of the nucleus via the visualisation of the mitotic spindle, whilst RAB11 was found to be a useful marker to track flagellar pocket division and follow mitochondrial DNA (kinetoplast) segregation. Classification and quantification of these morphological events were used to determine the durations of phases of the cell cycle. Our results demonstrate that in L. major promastigotes the extrusion of the daughter flagellum precedes the onset of mitosis, which in turn ends after kinetoplast segregation, and that significant remodelling of cell shape accompanies mitosis and cytokinesis. These findings contribute to a more complete foundation for future studies of cell cycle control in Leishmania.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Coombs, Professor Graham and Mottram, Professor Jeremy
Authors: Ambit, A., Woods, K. L., Cull, B., Coombs, G. H., and Mottram, J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Eukaryotic Cell
Publisher:American Society for Microbiology
ISSN:1535-9778
ISSN (Online):1535-9786
Published Online:16 September 2011
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
454141Analysing the roles of petidases in Leishmania infectivity and pathogenicityJeremy MottramMedical Research Council (MRC)G0700127Infection Immunity and Inflammation Life Sciences