Confocal and multiphoton imaging of intracellular Ca²⁺

Smith, G. , Reynolds, M., Burton, F.L. and Kemi, O.J. (2010) Confocal and multiphoton imaging of intracellular Ca²⁺. Methods in Cell Biology, 99(10), pp. 255-261. (doi: 10.1016/B978-0-12-374841-6.00009-8)

Abstract

This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca²⁺ within living cells. In particular, the excitation spectra of popular fluorescent Ca²⁺ indicators are shown during 1P and 2P excitation. The strengths and limitations of the current indicators are discussed along with error analysis which highlights the value of matching the Ca²⁺ affinity of the dye to a particular aspect of Ca²⁺ signaling. Finally, the combined emission spectra of Ca²⁺ and voltage sensitive dyes are compared to allow the choice of the optimum combination to allow simultaneous intracellular Ca²⁺ and membrane voltage measurement.

• Item Type: Articles
• Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in <i>Methods in Cell Biology</i>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in <i>Methods in Cell Biology</i>, [99, 10, (2010)] DOI: 10.1016/B978-0-12-374841-6.00009-8
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Smith, Professor Godfrey and Kemi, Dr Ole and Burton, Dr Francis
• Authors: Smith, G., Reynolds, M., Burton, F.L., and Kemi, O.J.
• Subjects: Q Science > QH Natural history > QH301 Biology
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Methods in Cell Biology
• Publisher: Academic Press
• ISSN: 0091-679X
• Copyright Holders: Copyright © 2010 Elsevier Inc
• First Published: First published in Methods in Cell Biology 99(10):255-261
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

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