Confocal and multiphoton imaging of intracellular Ca2+

Smith, G. , Reynolds, M., Burton, F.L. and Kemi, O.J. (2010) Confocal and multiphoton imaging of intracellular Ca2+. Methods in Cell Biology, 99(10), pp. 255-261. (doi: 10.1016/B978-0-12-374841-6.00009-8)



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This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca<sup>2+</sup> within living cells. In particular, the excitation spectra of popular fluorescent Ca<sup>2+</sup> indicators are shown during 1P and 2P excitation. The strengths and limitations of the current indicators are discussed along with error analysis which highlights the value of matching the Ca<sup>2+</sup> affinity of the dye to a particular aspect of Ca<sup>2+</sup> signaling. Finally, the combined emission spectra of Ca<sup>2+</sup> and voltage sensitive dyes are compared to allow the choice of the optimum combination to allow simultaneous intracellular Ca<sup>2+</sup> and membrane voltage measurement.

Item Type:Articles
Additional Information:NOTICE: this is the author’s version of a work that was accepted for publication in <i>Methods in Cell Biology</i>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in <i>Methods in Cell Biology</i>, [99, 10, (2010)] DOI: 10.1016/B978-0-12-374841-6.00009-8
Glasgow Author(s) Enlighten ID:Smith, Professor Godfrey and Kemi, Dr Ole and Burton, Dr Francis
Authors: Smith, G., Reynolds, M., Burton, F.L., and Kemi, O.J.
Subjects:Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Methods in Cell Biology
Publisher:Academic Press
Copyright Holders:Copyright © 2010 Elsevier Inc
First Published:First published in Methods in Cell Biology 99(10):255-261
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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