Kutter, C., Brown, G.D., Gonçalves, Â., Wilson, M.D., Watt, S., Brazma, A., White, R.J. and Odom, D.T. (2011) Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes. Nature Genetics, 43(10), pp. 948-955. (doi: 10.1038/ng.906)
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Publisher's URL: http://dx.doi.org/10.1038/ng.906
Abstract
RNA polymerase III (Pol III) transcription of tRNA genes is essential for generating the tRNA adaptor molecules that link genetic sequence and protein translation. By mapping Pol III occupancy genome-wide in mouse, rat, human, macaque, dog and opossum livers, we found that Pol III binding to individual tRNA genes varies substantially in strength and location. However, when we took into account tRNA redundancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes, we discovered strong conservation. Similarly, Pol III occupancy of amino acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, although the usage of individual tRNA genes has evolved rapidly.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | White, Prof Robert |
| Authors: | Kutter, C., Brown, G.D., Gonçalves, Â., Wilson, M.D., Watt, S., Brazma, A., White, R.J., and Odom, D.T. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Nature Genetics |
| Publisher: | Nature Publishing Group |
| ISSN: | 1061-4036 |
| ISSN (Online): | 1546-1718 |
| Published Online: | 28 August 2011 |
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