Jantos-Siwy, J., Schiffer, E., Brand, K., Schumann, G., Rossing, K., Delles, C. , Mischak, H. and Metzger, J. (2008) Quantitative urinary proteome analysis for biomarker evaluation in chronic kidney disease. Journal of Proteome Research, 8(1), pp. 268-281. (doi: 10.1021/pr800401m) (PMID:19012428)
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Abstract
A limitation of proteomic methods with respect to their clinical applicability is the lack of possibilities to directly deduce the amount of a protein or peptide from a particular mass spectrometry (MS) spectrum. For quantification of chronic kidney disease (CKD)-specific urinary polypeptides in capillary electrophoresis coupled with mass spectrometry (CE-MS), we compared signal intensity calibration methods based on either urinary creatinine or stable isotope labeled synthetic marker analogues (absolute quantification) with those based on ion counting using highly abundant collagen fragments as nonmarker references (relative quantification). Our results indicate that relative quantification of biomarker excretion based on ion counts in reference to endogenous “housekeeping” peptides is sufficient for the determination of urinary polypeptide levels. The calculation of absolute concentrations via exogenous stable isotope-labeled peptide standards is of no additional benefit.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Schiffer, Dr Eric and Delles, Professor Christian and Mischak, Professor Harald |
| Authors: | Jantos-Siwy, J., Schiffer, E., Brand, K., Schumann, G., Rossing, K., Delles, C., Mischak, H., and Metzger, J. |
| Subjects: | R Medicine > R Medicine (General) Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Journal of Proteome Research |
| Publisher: | American Chemical Society |
| ISSN: | 1535-3893 |
| ISSN (Online): | 1535-3907 |
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