Herpes simplex virus type 1 regulatory protein ICP0 aids infection in cells with a pre-induced interferon response but does not impede interferon induced gene induction

Everett, R.D. and Orr, A. (2009) Herpes simplex virus type 1 regulatory protein ICP0 aids infection in cells with a pre-induced interferon response but does not impede interferon induced gene induction. Journal of Virology, 83(10), pp. 4978-4983. (doi: 10.1128/JVI.02595-08)

Abstract

Several independent lines of evidence indicate that interferon mediated innate responses are involved in controlling herpes simplex virus type 1 (HSV-1) infection, and that the viral immediate-early regulatory protein ICP0 augments HSV-1 replication in interferon-treated cells. However, this is a complex situation in which the experimental outcome is determined by choice of multiplicity of infection and cell type, and whether cultured cells or animal models are used. It is now known that neither STAT1 nor IRF-3 play essential roles in the replication defect of ICP0 null mutant HSV-1 in cultured cells. This study set out to investigate the specific role of ICP0 in HSV-1 resistance to the interferon defence. We have used a cell line in which ICP0 expression can be induced at levels similar to those during the early stages of a normal infection to determine whether ICP0 by itself can interfere with interferon or IRF-3 dependent signalling, and whether ICP0 enables the virus to circumvent the effects of interferon stimulated genes (ISGs). We found that the presence of ICP0 was unable to compromise ISG induction by either interferon or double stranded RNA. On the other hand, ICP0 pre-expression reduced but did not eliminate the inhibitory effects of ISGs on HSV-1 infection, with the extent of the relief being highly dependent on multiplicity of infection. The results are discussed in terms of the relationships between ICP0 and intrinsic and innate antiviral resistance mechanisms

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everett, Professor Roger
• Authors: Everett, R.D., and Orr, A.
• Subjects: Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Virology
• Journal Abbr.: J. Virol.
• ISSN: 0022-538X
• ISSN (Online): 1098-5514
• Published Online: 04 March 2009