Restriction of the felid lentiviruses by a synthetic feline TRIM5-CypA fusion

Dietrich, I., McEwan, W. A., Hosie, M. J. and Willett, B. J. (2011) Restriction of the felid lentiviruses by a synthetic feline TRIM5-CypA fusion. Veterinary Immunology and Immunopathology, 143(3-4), pp. 235-242. (doi: 10.1016/j.vetimm.2011.06.017)

Abstract

Gene therapy approaches to the treatment of HIV infection have targeted both viral gene expression and the cellular factors that are essential for virus replication. However, significant concerns have been raised regarding the potential toxic effects of such therapies, the emergence of resistant viral variants and unforeseen biological consequences such as enhanced susceptibility to unrelated pathogens. Novel restriction factors formed by the fusion of the tripartite motif protein (TRIM5) and cyclophilin A (CypA), or "TRIMCyps", offer an effective antiviral defence strategy with a very low potential for toxicity. In order to investigate the potential therapeutic utility of TRIMCyps in gene therapy for AIDS, a synthetic fusion protein between feline TRIM5 and feline CypA was generated and transduced into cells susceptible to infection with feline immunodeficiency virus (FIV). The synthetic feline TRIMCyp was highly efficient at preventing infection with both HIV and FIV and the cells resisted productive infection with FIV from either the domestic cat or the puma. Feline TRIMCyp and FIV infection of the cat offers a unique opportunity to evaluate TRIMCyp-based approaches to genetic therapy for HIV infection and the treatment of AIDS.

• Item Type: Articles
• Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Veterinary Immunology and Immunopathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Veterinary Immunology and Immunopathology 143(3-4), 2011. DOI: 10.1016/j.vetimm.2011.06.017
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hosie, Professor Margaret and Willett, Professor Brian and Dietrich, Dr Isabelle
• Authors: Dietrich, I., McEwan, W. A., Hosie, M. J., and Willett, B. J.
• Subjects: Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Veterinary Immunology and Immunopathology
• Publisher: Elsevier B.V.
• ISSN: 0165-2427
• ISSN (Online): 1873-2534|
• Published Online: 12 June 2011
• Copyright Holders: Copyright © 2011 Elsevier B.V
• First Published: First published in Veterinary Immunology and Immunopathology 143(3-4):235-242
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

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