Sumino, A. et al. (2011) Construction and Structural Analysis of Tethered Lipid Bilayer Containing Photosynthetic Antenna Proteins for Functional Analysis. Biomacromolecules, 12(7), pp. 2850-2858. (doi: 10.1021/bm200585y)
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Publisher's URL: http://dx.doi.org/10.1021/bm200585y
Abstract
The construction and structural analysis of a tethered planar lipid bilayer containing bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis. The planar lipid bilayer containing LH2 and/or LH1-RC complexes was successfully formed on an avidin-immobilized coverglass via an avidin-biotin linkage. Atomic force microscopy (AFM) showed that a smooth continuous membrane was formed there. Lateral diffusion of these membrane proteins, observed by a fluorescence recovery after photobleaching (FRAY), is discussed in terms of the membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane was observed by steady-state fluorescence spectroscopy, indicating that the tethered membrane can mimic the natural situation
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cogdell, Professor Richard and Gardiner, Dr Alastair |
| Authors: | Sumino, A., Dewa, T., Takeuchi, T., Sugiura, R., Sasaki, N., Misawa, N., Tero, R., Urisu, T., Gardiner, A.T., Cogdell, R.J., Hashimoto, H., and Nango, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Biomacromolecules |
| ISSN: | 1525-7797 |
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