Hassan, H. and Cooper, G. (2009) Determination of β-hydroxybutyrate in blood and urine using gas chromatography–mass spectrometry. Journal of Analytical Toxicology, 33(8), pp. 502-507. (doi: 10.1093/jat/33.8.502)
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Abstract
β-Hydroxybutyrate (BHB) is considered a potential biomarker for alcoholic ketoacidosis (AKA). A robust and sensitive method was developed and validated for the quantitative determination of BHB in postmortem blood and urine using deuterated γ-hydroxybutyrate as an internal standard. Samples were analyzed by gas chromatography–mass spectrometry following liquid–liquid extraction and silyl derivatization. The limits of detection and lower limits of quantification in blood and urine were 2 and 7 mg/L and 2 and 6 mg/L, respectively. The interday and intraday precision was measured by coefficients of variation for blood and urine and ranged from 1.0 to 12.4% for quality control samples spiked at 50 and 300 mg/L. The linear range of 50–500 mg/L resulted in an average correlation of R² > 0.99, and the average extraction recoveries in blood and urine were ≥ 82% and ≥ 59%, respectively. BHB remains stable in blood spiked at a concentration of 300 mg/L for 15 days when stored within a refrigerator (2–5°C). Postmortem blood and urine samples were analyzed using the validated method for cases where the deceased had a history of chronic alcohol abuse to establish the use of BHB as a potential marker of AKA.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hassan, Mrs Huda and Cooper, Dr Gail |
| Authors: | Hassan, H., and Cooper, G. |
| Subjects: | R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities |
| Journal Name: | Journal of Analytical Toxicology |
| Journal Abbr.: | JAT |
| Publisher: | Preston Publications |
| ISSN: | 0146-4760 |
| ISSN (Online): | 1945-2403 |
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